Why start with 10x objective

Once you have the object in focus, then switch to the next higher power objective. Low Power Objective 10x The low power objective lens has more magnification power than the scanning objective lens, and it is one of the most helpful lenses when it comes to observing and analyzing glass slide samples. You should always begin studying a slide on low power, because this makes it easiest to find objects on the slide.

Position the diaphragm so that the largest opening is used. This will allow the maximum amount of light to be used. Objective lenses Always start and end your microscope session by placing the lowest power objective lens in position. This will make it easier to prevent crashing the objective lens into the slide. In general, the more light delivered to the objective lens, the greater the resolution.

The size of the objective lens aperture opening decreases with increasing magnification, allowing less light to enter the objective. You will likely need to increase the light intensity at the higher magnifications. Light Intensity Decreases Image brightness is inversely proportional to the magnification squared. Given a fourfold increase in magnification, the image will be 16 times dimmer. The depth of focus is greatest on the lowest power objective. Once the specimen is in view under this magnification, it is also easier to focus on the object using the fine focus knob on the side of the microscope.

Also, by using the lowest magnification and thus shorter lens, the stage is able to be raised higher to bring the sample up closer to the eye piece. Once the specimen is in sight, a gradual increase in magnification can provide clearer details and a more close-up look at the specimen. When magnification is increased, the specimen must be recentered and refocused carefully.

This in turn makes it easier to focus on the sample. Why do you need to start with 4x in magnification on a microscope? The 4x objective lens has the lowest power and, therefore the highest field of view. As a result, it is easier to locate the specimen on the slide than if you start with a higher power objective. Begin typing your search term above and press enter to search. Press ESC to cancel. Skip to content Home Physics What is the working distance for a 10x objective lens?

Ben Davis April 20,